Fluorescein

Fluorescein
Fluorescein
Systematic name Fluorescein
Chemical formula C20H12O5
Molecular mass 332.32 g/mol
Density 1.602 g/mL
Melting point 314-316°C
Boiling point
CAS number [2321-07-5]
SMILES c1ccc2c(c1)C(=O)OC23c4ccc(cc4Oc5c3ccc(c5)O)O

Fluorescein (chemical formula C20H12O5) is a highly fluorescent substance, absorbing light mainly in the blue range and emitting light mainly in the green range. This substance and its derivatives are commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein is known as the color additive named D&C Yellow no. 7, and its disodium salt is called D&C Yellow no. 8.

Contents

Chemical and physical properties

Fluorescein under UV illumination.

Fluorescein has an absorption maximum at 494-nanometer (nm) wavelength, and an emission maximum of 521 nm (in water). It has an acid dissociation constant (pKa) at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. The wavelength of 460 nm is its "isosbestic point"—that is, absorption at this wavelength is equal for all pH values.

Also, the fluorescence lifetimes of the protonated and deprotonated forms of fluorescein are approximately three and four nanoseconds (ns). This property can be used to determine pH from non-intensity based measurements. The lifetimes can be recovered using time-correlated single photon counting or phase-modulation fluorimetry.

Fluorescein Isothiocyanate

There are many fluorescein derivatives, for example fluorescein isothiocyanate, often abbreviated as FITC. In the FITC molecule, an isothiocyanate group (-N=C=S) replaces a hydrogen atom on the bottom ring of the fluorescein molecule. This derivative is reactive towards amine groups on proteins inside cells. Other derivatives include Oregon Green, Tokyo Green, SNAFL, and carboxynaphthofluorescein. These have been tailored for various chemical and biological applications where higher photostability, different spectral characteristics, or different attachment groups are needed.

Synthesis

Fluorescein can be prepared from phthalic anhydride and resorcinol in the presence of zinc chloride (catalyst) via the Friedel-Crafts reaction.

ZnCl2 fluorescein.png

A second method to prepare fluorescein uses methanesulfonic acid as the catalyst.

Applications

Uses in river systems

Fluorescein used in the Chicago River.

One of Fluorescein's more recognizable uses is in the Chicago River, where fluorescein is used to dye the river green on St. Patrick's Day.

Other uses of fluorescein include using it as a water-soluble dye added to rainwater in environmental testing simulations to aid in locating and analyzing any water leaks, and in Australia and New Zealand as a methylated spirit dye.

Biological research

In biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence microscopy applications. Fluorescein may also be attached to biologically active molecules (such as antibodies), allowing biologists to target the fluorophore to specific proteins or structures within cells. This application is common in yeast display.

Fluorescein can also be linked to nucleoside triphosphates and incorporated into a probe for in situ hybridization. Fluorescein-labeled probes can be imaged using a technique known as FISH (fluorescent in situ hybridization), or targeted by antibodies using immunohistochemistry. The latter is a common alternative to digoxigenin, and the two are used together for labeling two genes in one sample.

Ophthalmic applications

Fluorescein in dropper used for eye examination.

Fluorescein sodium is used extensively as a diagnostic tool in the field of ophthalmology. It is applied topically in the form of a drop or it can be injected intravenously to produce a fluorescein angiogram.

Topical fluorescein is useful in the diagnosis of corneal abrasions, corneal ulcers, herpetic corneal infections, and dry eye. Fluorescein angiography is used to diagnose and categorize macular degeneration, diabetic retinopathy, inflammatory intraocular conditions, and intraocular tumors.

See also

References

  • Lakowicz, Joseph R. 2006. Principles of Fluorescence Spectroscopy. Second Edition. New York, NY: Springer Science. ISBN 0387312781
  • Stevens, Rosalind A., Patrick J. Saine, and Marshall E. Tyler. 1999. Stereo Atlas of Fluorescein and Indocyanine Green Angiography. 1st edition. Woburn, MA: Butterworth-Heinemann. ISBN 0750670010
  • Valeur, Bernard. 2001. Molecular Fluorescence: Principles and Applications. Weinheim, Federal Republic of Germany: Wiley-VCH Verlag GmbH. ISBN 352729919X

External links

All links retrieved April 14, 2017.

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